Extended Data Fig. 2: The mTORC2-deficiency impairs memory CD4+ T cell pool but enhances memory CD8+ T cell pool in acute viral infection. | Nature Immunology

Extended Data Fig. 2: The mTORC2-deficiency impairs memory CD4+ T cell pool but enhances memory CD8+ T cell pool in acute viral infection.

From: The kinase complex mTORC2 promotes the longevity of virus-specific memory CD4+ T cells by preventing ferroptosis

Extended Data Fig. 2

a, b, Analysis of TH1 (SLAMhighCXCR5− in a, CXCR5− in b) and TFH (SLAMlowCXCR5+ in a, CXCR5+ in b) cells among viral-specific memory CD4+ T (positive for LCMV GP66-77 tetramer) cells in the spleens of either Rictorfl/fl (blue) or CD4Cre-Rictorfl/fl (Rictor−/−, red) mice at day 8 (a) and day 100 (b) post-infection of LCMV Arm+. The presented data are representative of three independent experiments (n=6 for Rictorfl/fl and n=4 for Rictor−/− in a, n=6 for both groups in b). c, Viral-specific memory CD8+ T (positive for LCMV GP33-41 tetramer) cells in the spleens of either Rictorfl/fl (blue) or CD4Cre-Rictorfl/fl (Rictor−/−, red) mice. The presented data are representative of three independent experiments (n=4). d, Ratios of total CD8+ T cells and virus-specific CD8+ T cells (positive for LCMV GP33-41 tetramer) between ERT2Cre-Rictorfl/fl origin (CD45.2+) and wild-type origin (CD45.1+) in the spleens of bone marrow chimeras (BMCs) after administration of Tamoxifen (red) or vehicle (blue) at the memory maintenance stage as described in Fig. 2b, from three independent experiments (n=4). Data are presented as mean values ± SD and analyzed by unpaired two-tailed t-test in a-d.

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