Extended Data Fig. 3: Torin-1 induced mTORC2-deficiency impairs the virus-specific memory CD4+ T cell pool in vivo.
a, Experimental setup of in vivo administration of Torin-1, Rapamycin or vehicle on adoptively transferred memory SMARTA cells (CD45.1+) in recipients (CD45.2+). b, p-AKTSer473 levels on transferred SMARTA cells in spleens of recipients as in a. The presented data are representative of three independent experiments. c, p-S6Ser235/236 levels on memory SMARTA cells in the presence or absence of αCD3 plus αCD28 ex vivo in spleens of recipients. The presented data are representative of three independent experiments (n=4). d, Frequency change of memory SMARTA cells in PBMCs after administration of either Torin-1, Rapamycin, or vehicle in recipients as in a. The presented data are representative of three independent experiments (n=5). Grey area in histogram indicates staining with rabbit isotype IgG in b-c. Data are presented as mean values ± SD and analyzed by unpaired two-tailed t-test in c. Data are analyzed by paired two-tailed t-test in d.
