Extended Data Fig. 4: Whole blood CyTOF and flow cytometry analysis strategy. | Nature Immunology

Extended Data Fig. 4: Whole blood CyTOF and flow cytometry analysis strategy.

From: Prolonged activation of nasal immune cell populations and development of tissue-resident SARS-CoV-2-specific CD8+ T cell responses following COVID-19

Extended Data Fig. 4

Pre-processed CyTOF files were clustered using hierarchical SNE (hSNE) and Gaussian mean shift clustering in Cytosplore software. a) Landmark clustering of overview level, with color corresponding to clusters. b) Heatmap showing marker expression, used for annotation of the lineages. c) Clustering of all CD4 + T cells in level 2, with landmarks colored by cluster. d) Clustering of memory CD4 + T cells, with colors corresponding to clusters in level 3. e) Clustering of CD4 + T EM cells on the data level, with cells colored per cluster. pDC = plasmacytoid dendritic cell. ILC = innate lymphoid cells. NK = natural killer. CM = central memory. EM = effector memory. EMRA = effector memory re-expressing CD45RA.

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