Extended Data Fig. 7: Gating strategy and analysis of the CD11b + myeloid cells in CNS and sdLN of mixed BM chimeras during EAE; marker expression of Ccr2Ai14 CD11b + cells at peak EAE and targeting of Ccr2CreERT2xIabfl in sdLNs during EAE induction. | Nature Immunology

Extended Data Fig. 7: Gating strategy and analysis of the CD11b + myeloid cells in CNS and sdLN of mixed BM chimeras during EAE; marker expression of Ccr2Ai14 CD11b + cells at peak EAE and targeting of Ccr2CreERT2xIabfl in sdLNs during EAE induction.

From: IFNγ and GM-CSF control complementary differentiation programs in the monocyte-to-phagocyte transition during neuroinflammation

Extended Data Fig. 7

a, Gating strategy for the myeloid cells in sdLN and CNS of MOG35-55 immunized mice before dimensionality reduction and clustering. from the CNS of Csf2rb + /+ (CD45.1+): Csf2rb−/− (CD45.2+) and Ifngr1 + /+(CD45.1+): Ifngr1−/− (CD45.2+) BM chimeras. b, Median expression of the markers selected to define MdC populations, overlaid to the UMAP of CNS Mdcs from Ccr2Ai14 mice at peak EAE (14 d.p.i.). c, Representative plots are shown for: CD45 + Ly6G-CD11b + Ly6C+moDCs +and CD45 + Ly6G-Ly6C-CD11b + CD11c + CD26 + XCR1 + cDCs. Data represents 2 independent experiments (n = 5 per group), p values were calculated using an unpaired two-tailed t test with Welch’s correction. **p < 0.0079. Data are shown as means ± SEM. n.s., not significant.

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