Fig. 1: Steady-state DETC–KC interactions depend on the Vγ5Vδ1 TCR and Skint1.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a,b, Confocal microscopy of CD3+ DETCs (cyan) and F-actin within a CD3 mask (yellow) in epidermal sheets. The frequency distribution of the number of interacting dendrites (CD3- and F-actin-rich aggregates) per cell is shown, using mean values per mouse. Interacting dendrites from wild-type FVB (n = 16), FVB.Tac (n = 12), Trgv5–/–Trdv4–/– (n = 7) and Skint1-transgenic FVB.Tac mice (n = 12) are shown in a; scale bar, 20 μm. Interacting dendrites from wild-type FVB mice administered Skint1 antibody (n = 10) or isotype control (n = 9) by intradermal (i.d.) injection 6 h before are shown in b; scale bar, 20 μm. c, Instant structured illumination microscopy (iSIM) of whole-mount epidermal sheets from unchallenged wild-type FVB mice. Left, Vγ5 TCR (green) staining; scale bar, 10 μm. Right, top–down and lateral views of three-dimensional (3D) reconstructions of typical Vγ5 TCR (green) and Skint1 (magenta) staining in selected dendrite tips from the left-hand image; scale bar, 1 μm. Representative images and pooled data are from eight (a) or three (b and c) independent experiments. A matched two-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test relative to wild-type (a) or a Sidak’s multiple comparisons test (b) was used to analyze the data. Values in pie charts represent mean ± s.d.; NS, not significant.
