Extended Data Fig. 5: DETC stress surveillance via TNF superfamily receptors is licensed by Skint1.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a) qPCR for gene expression (DETCs/LCs n=4, KCs n=3) and b) flow cytometry for surface expression of 4-1BB and GITR in epidermal cell populations from unchallenged wild-type C57BL/6 mice. Representative of n=3 mice (b). c) qPCR for DETC gene expression in epidermis at 24hr post-UVR from wild-type C57BL/6 (n=14) and Tnfsf9-/- mice (n=15). d) Tnfrsf18 expression in steady state DETC clusters identified from scRNAseq as in Fig. 3. Pooled data from 3 independent experiments (c). Paired two-way ANOVA with Sidak’s multiple comparisons test (c). Error bars represent mean ± SD.
