Extended Data Fig. 6: Skint1-dependent DETC responses promote epithelial recovery from DNA damage.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a) Wild-type C57BL/6 mice were administered Skint1 Ab or isotype control by intradermal injection 24hr prior to and 48hr post-ultraviolet radiation (UVR) of dorsal ear skin. Representative confocal microscopy images of γH2A.X nuclei (magenta) and CD3+ DETCs (cyan) in ear sheets, as quantified in Fig. 6b at d3 post-UVR. Righthand images overlay F-actin staining (yellow). Scale bar 50μm (n=10/group). Relative increase in ear thickness at 5d post-UVR relative to unchallenged ear in b) wild-type C57BL/6 (n=6) vs Tcrd-/- mice (n=5), c) littermate control (n=10) vs Skint1-/- mice (n=10) and d) littermate control (n=4) vs Skint locus-/- mice (n=5). Representative of (a) or pooled data from (c) 2 independent experiments. Unpaired two-tailed t-test (b-d) with Welch’s correction where necessary (b-c). Error bars represent mean ± SD.
