Fig. 2: Skint1 underpins maintenance of epidermal integrity by DETCs.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a, Flow cytometric quantification of Vγ5Vδ1+ DETCs and LCs in wild-type FVB mice administered Skint1 antibody or isotype control by i.d. injection into the ear weekly for 10 weeks (n = 8 per group). b, Total ear thickness at 10 weeks (n = 8 per group) in mice treated as in a. c, Hematoxylin and eosin (H&E) sections of injected ear skin and quantification of epidermal thickness at 5 weeks in mice treated as in a (n = 5 per group); scale bar, 100 μm. d, Flow cytometric quantification of epidermal CD3+CD5+TCRβ+ T cells in mice treated as in a (n = 10 per group). e, Transepidermal water loss from ear skin in wild-type (n = 8 per group) and Tcrd–/– mice (n = 7 per group) administered Skint1 antibody or isotype control by i.d. injection into the ear weekly for 5 weeks. f, Quantitative PCR (qPCR) of Il13 expression in the epidermis of wild-type mice treated as in e (n = 8 isotype control; n = 10 Skint1 antibody). g, qPCR of KC gene expression in the epidermis of mice treated as in a (n = 8 per group). h, Flow cytometric analysis of viability and apoptosis of CD45– KCs in mice treated as in a (n = 8 per group); IR, infrared. i, Flow cytometry of activated LC frequency in mice treated as in a (n = 8 per group). Data are representative of (a–c, h and i) or are pooled from (d–g) at least two independent experiments per time point. A paired two-way ANOVA with Sidak’s multiple comparisons test (b), an unpaired two-sided t-test (c, f–i), an unpaired two-sided t-test with Welch’s correction (d) or an ordinary two-way ANOVA with Tukey’s multiple comparisons test (e) was used to analyze the data. Error bars represent mean ± s.d.
