Fig. 3: Peripheral Skint1 sustains steady-state DETC signatures of immunosurveillance.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a, UMAP of the combined single-cell RNA-sequencing dataset of DETCs from wild-type FVB mice administered Skint1 antibody or isotype control by i.d. injection 1 week before (n = 1 per group). b, Top 10 most significantly enriched genes per cluster in DETCs from the combined dataset, as in a. c, Expression of key cluster 3 marker genes in the combined dataset, as in a. d, Flow cytometry expression of green fluorescent protein (GFP) in DETCs segregated by 4-1BB expression from Nur77–GFP mice treated 2 d prior with Skint1 antibody or isotype control by i.d. injection (n = 5 per group); gMFI, geometric mean fluorescence intensity. e, UMAP of DETCs comparing treatments, as in a. f, Contribution of DETCs from Skint1 antibody- and isotype-treated samples to cluster composition, as in a. g, Expression of Xcl1 in DETCs, comparing Skint1 antibody and isotype treatments, as in a. h, Differential gene expression in combined DETC clusters comparing Skint1 antibody and isotype treatments, as in a; FMO, fluorescence minus one i, Frequency of 4-1BBhi DETCs by flow cytometry in wild-type FVB mice treated with Skint1 antibody or isotype control by i.d. injection 1 week prior (n = 10 per group). j, Gene set enrichment analysis (GSEA) of combined DETC clusters comparing Skint1 antibody and isotype treatments, as in a, using a Skint1 selection gene signature from DETC precursors isolated from wild-type or FVB.Tac embryonic thymi; NES, normalized enrichment score; FDR, false discovery rate. Data are representative of three independent experiments (d) or are pooled from two independent experiments (i). A Wilcoxon test with Bonferroni’s correction (b and h), paired one-way ANOVA with Sidak’s multiple comparisons test (d) or unpaired two-sided t-test (i) was used to analyze the data. Error bars represent mean ± s.d.
