Fig. 5: DETC stress surveillance via TNF superfamily receptors is licensed by Skint1.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a,b, Flow cytometry (a) of DETC CD69 and GFP expression and qPCR (b) for DETC gene expression in the epidermis from Nur77–GFP reporter mice administered agonistic 4-1BB antibody or isotype control 24 h following Skint1 antibody or isotype control administration by i.d. injection and analyzed 6 h later (n = 4 isotype/isotype; n = 3 Skint1 antibody/isotype; n = 7 isotype/4-1BB antibody; n = 7 Skint1 antibody/4-1BB antibody). c, qPCR for DETC gene expression in the epidermis at 24 h after UVR from wild-type C57BL/6 mice pretreated with 4-1BBL antibody or isotype control by i.d. injection (n = 10 per group). d, Flow cytometry of GFP and CD69 expression in DETCs from Nur77–GFP mice administered agonistic glucocorticoid-induced TNF receptor-related protein (GITR) antibody or isotype control 24 h after i.d. injection with Skint1 antibody or isotype control and analyzed 6 h later (n = 5 isotype/isotype; n = 5 isotype/GITR antibody; n = 4 Skint1 antibody/GITR antibody). e, qPCR of DETC gene expression in the epidermis at 24 h after UVR from wild-type C57BL/6 mice pretreated with GITRL antibody or isotype control by i.d. injection (n = 10 per group). f, Flow cytometry of GFP and CD69 expression in DETCs from Nur77–GFP mice administered ATP or PBS 24 h after i.d. injection with Skint1 antibody or isotype control and analyzed 9 h later (n = 5 per group). Data are pooled from two independent experiments (a–f). An ordinary (a and b) or paired (c, e and f) two-way ANOVA with Sidak’s multiple comparisons test was performed or an ordinary one-way ANOVA with Tukey’s multiple comparisons test (d). Error bars represent mean ± s.d.
