Fig. 6: Skint1-dependent DETC responses promote epithelial recovery from DNA damage.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a, Enzyme-linked immunosorbent assay (ELISA) for CPDs in epidermal DNA extracts from wild-type C57BL/6 mice administered Skint1 antibody or isotype control by i.d. injection 24 h before and 48 h after UVR of dorsal ear skin (n = 10 per group). b, Confocal microscopy quantification of γH2A.X nuclei in ear sheets per field of view (FOV) at day 3 after UVR in mice treated as in a (n = 10 per group; mean value reported per mouse). c, Total ear thickness at day 5 after UVR compared to that observed in contralateral unirradiated ears in mice treated as in a (isotype, n = 13; Skint1 antibody, n = 14). d, H&E histology of ear sections and quantification of mean epidermal thickness at day 5 after UVR compared to the non-irradiated ventral side in mice treated as in a; scale bar, 100 μm (n = 3 per group). e, Toluidine blue permeation of ears at day 5 after UVR in mice treated as in a (n = 7 per group). Data are pooled from two (a, b and e) or three (c) independent experiments or are representative of two independent experiments (d). Paired two-way ANOVA with Sidak’s multiple comparisons tests (a and c–e) or an unpaired two-sided t-test (b) was used to analyze the data. Error bars represent mean ± s.d.
