Fig. 7: Skint1 licenses DETC responses to irritant contact dermatitis.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a, qPCR for effector gene expression in sorted DETCs at 48 h after treatment from wild-type C57BL/6 mice administered isotype control or Skint1 antibody by i.d. injection before treatment of ear skin with 0.5% DNFB solution or vehicle (isotype/isotype, n = 11; isotype/DNFB, n = 16; Skint1 antibody/DNFB, n = 13). b, Flow cytometry of GFP expression by DETCs from Nur77–GFP reporter mice at 48 h after treatment as in a (isotype, n = 7; Skint1 antibody, n = 6). c, Total ear thickness at 48 h after treatment as in a (isotype/isotype, n = 8; isotype/DNFB, n = 12; Skint1 antibody/DNFB, n = 10). Data are pooled from two (b and c) or three (a) experiments. An ordinary one-way ANOVA with Tukey’s multiple comparisons test (a and c) or paired two-way ANOVA with Sidak’s multiple comparisons test (b) was used to analyze the data. Error bars represent mean ± s.d.
