Extended Data Fig. 2: Skint1 underpins maintenance of epidermal integrity by DETCs.
From: Normality sensing licenses local T cells for innate-like tissue surveillance
a) Flow cytometry of epidermal CD45+ cellular composition and b) canonical TCR expression by DETCs from wild-type FVB mice administered with Skint1 Ab or isotype control by intradermal injection into the ear weekly for 10 weeks (n=8/group). c) Confocal microscopy of Vγ5+ DETCs (green) and MHC-II+ LCs (white) from mice treated as in a. Representative of n=8/group, scale bar 50 μm. d) Total ear thickness of wild-type FVB (n=5/group) or Tcrd-/- mice (n=4/group) administered with Skint1 Ab or isotype control by intradermal injection into the ear weekly for 5 weeks. e) Epidermal thickness measured from H&E sections of ear skin of mice treated as in d. f) Transepidermal water loss (TEWL) of injected and contralateral ear skin of wild-type mice treated as in d. g) Baseline TEWL in C57BL/6 Skint1+/- (n=7), Skint1-/- (n=7) and Skint locus-/- mice (n=5). h) qPCR of Il13 expression in epidermis of untreated wild-type FVB (n=14) and Tcrd-/- mice (n=9). i) qPCR of keratinocyte gene expression in epidermis of untreated wild-type FVB (n=15) and Tcrd-/- mice (n=9). j) qPCR of keratinocyte gene expression in epidermis of mice treated as in a (n=8/group). k) Baseline TEWL in wild-type FVB (n=18), Langerin-DTA (n=11) or Tcrd-/- mice (n=6). l) qPCR of keratinocyte gene expression in epidermis of wild-type FVB (n=7) and Langerin-DTA mice (n=5). Data representative of at least 2 independent experiments (a-b, d, f, k-l) or pooled from 3 (h-i) 2 (j) independent experiments. Ordinary two-way ANOVA with Tukey’s multiple comparisons test (d-e), ordinary one-way ANOVA with Dunnett’s multiple comparisons test relative to Skint1+/- (g), paired two-way ANOVA with Sidak’s multiple comparisons test (f), unpaired two-tailed t-test (h-j, l) or ordinary one-way ANOVA with Tukey’s multiple comparisons test (k). Error bars represent mean ± SD.
