Extended data Fig. 3: Effects of TKMG48 on PD-1 and CTLA-4 signals.
From: PD-1 agonism by anti-CD80 inhibits T cell activation and alleviates autoimmunity
a,b, Requirement of PD-1-signal for the TKMG48-induced inhibition of T cell activation. Expression of PD-1 and its mutants on DOdP T cells (a). DOdP T cells expressing PD-1 or its mutants were stimulated with pOVA323-339 (0.3 μM) using IIAdL cells expressing indicated molecules as APCs in the presence or absence of TKMG48 (10 μg ml−1). c, No substantial effect of TKMG48 on CTLA-4 signal. DOdP or DOdP-CTLA-4 T cells were stimulated with IIAdL-CD80 or IIAdL-CD80:CD86 cells pulsed with pOVA323-339 (0.3 µM) in the presence of TKMG48 (10 µg ml−1). IL-2 concentrations of the culture supernatants are shown (b,c). Representative data of 2 (a,b) and 3 (c) independent experiments are shown.
