Extended data Fig. 4: Dose-dependent effects of PD-L1, CD80, and PD-1 on TKMG48-induced T cell suppression.
From: PD-1 agonism by anti-CD80 inhibits T cell activation and alleviates autoimmunity
a–d, Dose-dependent effects of PD-L1 and CD80 on TKMG48-induced T cell suppression. Expression of PD-L1 and CD80 at high, intermediate (int), and low levels on IIAdL cells (a). PD-1-EC-binding intensities of IIAdL cells expressing PD-L1 and CD80 at varying levels in the presence or absence of TKMG48 (10 μg ml−1) (b). TKMG48-induced PD-1-mediated inhibition of T cell activation in a manner dependent on the amount of PD-L1 on IIAdL cells (c,d). DOdP-PD-1 T cells were stimulated with pOVA323-339 (0.3 μM) using IIAdL cells expressing indicated molecules at indicated levels as APCs in the presence or absence of anti-PD-L1 Ab (2 μg ml−1) and TKMG48 (10 μg ml−1). e–g, Effects of PD-1 expression level on TKMG48-induced T cell suppression. Expression of PD-1 at high, int, and low levels on DOdP T cells (e). Inhibition of T cell activation in a manner dependent on the amount of PD-1 on T cells (f). PD-1-mediated inhibition of T cell activation by TKMG48 in a manner dependent on the amount of PD-1 on T cells (g). DOdP T cells expressing PD-1 at indicated level were stimulated with pOVA323-339 (0.3 μM) using IIAdL cells expressing indicated molecules as APCs (f,g). TKMG48 or Ctrl Ig (10 µg ml−1) was added as indicated (g). IL-2 concentrations of the culture supernatants (c,f,g) and the percentages of inhibition (d) are shown. Representative data of 3 independent experiments are shown. Error bars denote SEM.
