Extended Data Fig. 2: ISR in macrophage activation and metabolism.
From: PERK is a critical metabolic hub for immunosuppressive function in macrophages
a,b, Expression of CD206, CD301 (a), PD-L2 and Relmα (b) in macrophages stimulated with IL-4 in the presence or absence of ISRIB (n = 3; mean ± s.e.m). Data is representative of three independent experiments. c, Representative histogram (left) and quantitative analysis (right) of iNOS expression in macrophages stimulated with LPS + IFN-γ in the presence or absence of ISRIB (n = 3; mean ± s.e.m). Data is representative of three independent experiments. d, Expression of genes associated with ISR, assessed by RNA-Seq analysis. e,f, Expression of CD206, CD301 (e), PD-L2 and Relmα (f) in Gcn2+/+ and Gcn2-/- macrophages in the presence or absence of IL-4 (n = 3; mean ± s.e.m). Data is representative of two independent experiments. g,h, Representative histograms (left) and quantitative analysis (right) of iNOS (g) or TNF-α (h) in Gcn2+/+ and Gcn2-/- macrophages in the presence or absence of LPS + IFN-γ (n = 3; mean ± s.e.m). Data is representative of two independent experiments. i,j, Basal OCR (i) and ECAR (j) of Gcn2+/+ and Gcn2-/- M0, M1, and M2 macrophages (n = 3; mean ± s.e.m). Data is representative of two independent experiments. k, Basal OCR of M0 and M2 BMDMs in the presence or absence of ISRIB (n = 3; mean ± s.e.m). Data is representative of two independent experiments. l, Intracellular serine content from M0, M1 and M2 macrophages in the presence of absence of ISRIB (n = 3; mean ± s.e.m). Data is representative of two independent experiments. All data were analyzed using two-tailed unpaired Student’s t-test (a,b,c,e,f,g,h,I,j,k,l).
