Extended Data Fig. 5: Qpctl loss in both hematopoietic or stromal compartments compromises monocytes but not lymphocytes or tissue macrophages.
a, Identification of tissue macrophages in liver, lung and brain sections from naive Qpctl+/+ and Qpctl–/– littermates. Scale is 100µM. Representative images of 5 mice per genotype (experiment done once). b, Leukocyte quantification in blood from Qpctl+/+ and Qpctl–/– littermates (n = 16 (Qpctl+/+) or 19 (Qpctl–/–)). NS, P > 0.05. c, Quantification of splenic patrolling monocytes (CD11b+CD115+Ly6C–CX3CR1+) in Qpctl+/+ and Qpctl–/– littermates (n = 10 (Qpctl+/+) or 9 (Qpctl–/–)). *P = 0.03. d,e, Chimeras were generated by reconstituting lethally irradiated WT or Qpctl–/– mice with WT or Qpctl–/– hematopoietic progenitors. d, Monocyte quantification in blood (n = 17 (WT→WT), 19 (Qpctl–/–→Qpctl–/–) or 20 (WT→Qpctl–/–, Qpctl–/–→WT) and spleen (n = 19 (WT→WT, Qpctl–/–→Qpctl–/–) or 20 (WT→Qpctl–/–, Qpctl–/–→WT). *P < 0.0001. e, Quantification of CCL7 PTMs in plasma (Total CCL7, n = 18 (WT→WT, Qpctl–/–→Qpctl–/–) or 19 (WT→Qpctl–/–, Qpctl–/–→WT); pE-CCL7, n = 19 (Qpctl–/–→Qpctl–/–, WT→Qpctl–/–) or 20 (WT→WT, Qpctl–/–→WT); ∆QP-CCL7, n = 19 (Qpctl–/–→Qpctl–/–, Qpctl–/–→WT) or 20 (WT→WT, WT→Qpctl–/–). NS, P > 0.05, *P = 0.0025, **P = 0.001, ***P = 0.0008, ****P < 0.0001. f, WT (CD45.1) and Qpctl–/– (CD45.2) monocytes were co-transferred into WT (CD45.1/CD45.2) hosts. Quantification of transferred monocytes into the peritoneal cavity following pE-CCL7 injection, normalized to the ratio of WT and Qpctl–/– monocytes transferred (n = 9). g, Quantification of splenic monocytes in Qpctl+/+ and Qpctl–/– littermates treated with Ctrl chow or DPP4i (n = 10). *P = 0.0002. h, Quantification of monocytes in blood and spleen of WT, Dpp4–/–, Qpctl–/– and Dpp4–/–Qpctl–/– littermates (n = 10 (Dpp4–/–) or 9 (WT, Qpctl–/– and Dpp4–/–Qpctl–/–)). *P = 0.0056, **P < 0.0001. Bars are medians and symbols represent individual mice. Data shown is representative (f), or pooled from 2-3 experiments (b,c,d,e,g,h). All experiments were repeated (≥ twice) besides a. Statistical analysis was done using unpaired, nonparametric Mann-Whitney U-test.
