Extended Data Fig. 6: Characterization of EO771 and LL/2 mouse tumor models.
a, Quantification of Ccl2, Ccl7 and Qpctl RNA expression in LL/2 and EO771 cells. (n = 2 (LL/2) or 1 (EO771) sequenced bulk samples, bars represent means). b, WT mice were inoculated with EO771 (n = 6) or with LL/2 (n = 8) cells. Frequency of tumor-associated leukocytes was quantified at day 14. Bars are medians and symbols individual mice. c, Gating strategy for the identification of leukocytes in tumors. d,e, Bulk CRISPR/Cas9-edited Qpctl–/– LL/2 and EO771 cells were generated. d, Quantification of pE-CCL7 in the cell supernatants, plotted as percentage of total CCL7, measured in the same samples. e, In vitro growth curves of tumor cell lines (n = 2 (EO771) or 1 (LL/2) technical replicates). ∅ - parental cell line; ntc - non-targeted control. Lines are means. Data shown are representative experiments. All experiments were repeated independently (≥ twice), except a, which was done once.
