Extended Data Fig. 1: Monocyte migration is not modulated by DPP4.
a, Quantification of MCPs in plasma of naive WT mice (n = 5). b, NH2-terminal amino acid sequence of MCPs highlighting the presence of a glutamine (Q) in the first position and a proline (P) in the second position. c, Monocyte quantification in blood (n = 5), spleen (n = 4 (+/+) or 5 (–/–)) and bone marrow (n = 4 (+/+) or 5 (–/–)) of naive Dpp4+/+ and Dpp4−/− littermates. NS, P > 0.05. d, Monocyte quantification in tumors extracted from WT mice treated with Ctrl chow or DPP4i (Hepa1-6, n = 11 (Ctrl) or 9 (DPP4i); CT26 (n = 11); B16F10 (n = 10); LL/2 (n = 10)). NS, P > 0.05. e, Human (h)CCL7 was incubated in the absence or presence of hDPP4. Molecular weight profiles of resulting species are shown in Daltons (Da). f, Schematic representation of antibodies that detect MCP forms: anti-pE recognizes NH2-terminal cyclization and anti-ΔQP is specific for the truncated chemokine. Anti-total antibody recognizes the chemokine independently of its N-terminal modification. g, Cross-reactivity profile of anti-mouse (m)CCL7 capture antibodies against mCCL7 forms. AF456 recognizes mCCL7 independently of its N-terminal modification (total); 1G2 binds preferentially to pE-CCL7 and 2F3 binds preferentially to ΔQP-CCL7. Detection was done with biotinylated anti-mCCL7 AF456. AEB, average enzyme per bead. h, Cross-reactivity profile of mCCL2 capture antibodies against mCCL7 and mCCL2 forms. Detection was done with biotinylated anti-mCCL2 MAB479. i, Lower limit of detection (LLOD) of antibodies used to analyze mCCL2 and mCCL7 PTMs. Bars are medians and symbols represent individual mice. Data shown is representative (a,c,g,h) or pooled from 2 experiments (d). All experiments were repeated independently (≥ twice). Statistical analysis was done using unpaired, nonparametric Mann-Whitney U-test.
