Extended Data Fig. 3: Visualizing cpLEC trajectories. | Nature Immunology

Extended Data Fig. 3: Visualizing cpLEC trajectories.

From: Neuroinflammation creates an immune regulatory niche at the meningeal lymphatic vasculature near the cribriform plate

Extended Data Fig. 3

(a): Single cell data was clustered using UMAP methodology and trajectories learned using default parameters by monacle3. Trajectories through pseudotime are shown for the three clusters; note that cluster 3 lies in between clusters 1 and 2 through pseudotime, and there are no direct connections between clusters 1 and 2. (b): 5 representative genes associated with the GO enrichment term adhesion/chemotaxis that is enriched in cluster 2 are shown through pseudotime. (c): 4 representative genes associated with the GO enrichment term response to IFN-γ that is enriched in cluster 2 are shown through pseudotime. (d): 3 representative genes associated with the GO enrichment term antigen processing/presentation that is enriched in cluster 2 are shown through pseudotime. (e): 4 representative genes associated with the GO enrichment term leukocyte activation that is enriched in cluster 2 are shown through pseudotime. Note that these genes tend to be up-regulated later through pseudotime relative to genes associated with the other 3 enrichment terms.

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