Extended Data Fig. 4: B cell-derived acetylcholine effects in the spleen.
From: B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis
a. Membranes of the cytokine antibody array of the spleen, serum and bone marrow of naive Cd19CreChatfl/fl mice or their Chatfl/fl littermates. Membranes represent two independently performed experiments per group. b. Left, flow cytometry for splenic HSPC. Right, splenic HSPC enumeration in naive Cd19CreChatfl/fl or Chatfl/fl mice. P = 0.036 for SLAM LSK, P = 0.040 for LSK. n = 7 mice per group, two-tailed Student’s t-test. c. Leukocytes in spleen. P = 0.017 for monocytes, P = 0.038 for neutrophils. n = 7 mice per group, two-tailed Mann-Whitney test. d. Vascular permeability was analyzed by Evans blue extraction and quantification in the spleen of Cd19CreChatfl/fl mice or Chatfl/fl mice. P = 0.0455. n = 5 mice per group, two-tailed Student’s t-test. Data are mean ± SEM with individual values for data distribution, *P < 0.05.
