Extended Data Fig. 5: Splenectomy and hematopoiesis.
From: B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis
a. Cd19CreChatfl/fl mice or their Chatfl/fl littermates underwent splenectomy or sham-operation and the bone marrow was harvested 4 weeks later for flow cytometry and cytokine/chemokine antibody array analysis. Membranes of the cytokine/chemokine antibody array of the bone marrow supernatant of splenectomized or sham-operated Cd19CreChatfl/fl mice or their Chatfl/fl littermates. Membranes represent two independently performed experiments per group. b. Flow cytometry plots for HSPC in the femur. c. Bone marrow HSPC enumeration in the femur. SLAM LSK: P = 0.0040 for sham, P = 0.0051 for splenectomy, GMP: P = 0.0003 for sham, P = 0.0108 for splenectomy, CMP: P = 0.0071 for sham, P = 0.0037 for splenectomy. Two independent experiments, n = 8 Chatfl/fl mice, sham Cd19CreChatfl/fl mice and splenectomy Chatfl/fl mice; n = 9 splenectomy Cd19CreChatfl/fl mice, two-tailed Student’s t-test. d. Flow cytometry plots for BrdU incorporation. e. Bone marrow HSPC proliferation analyzed by BrdU incorporation using flow cytometry. SLAM LSK: P = 0.0012 for sham, P = 0.0068 for splenectomy, GMP: P = 0.0034 for sham, P = 0.019 for splenectomy. Two independent experiments, n = 6 sham Chatfl/fl mice, n = 8 sham Cd19CreChatfl/fl mice, n = 7 splenectomy Chatfl/fl mice; n = 10 splenectomy Cd19CreChatfl/fl mice, two-tailed Student’s t-test. Data are mean ± SEM with individual values for data distribution, *P < 0.05, **P < 0.01 and ***P < 0.001.
