Extended Data Fig. 1: Acetylcholine and hematopoiesis.
From: B lymphocyte-derived acetylcholine limits steady-state and emergency hematopoiesis
a. 2,687 patients received either no donepezil (n = 870), 5 mg (n = 996) or 10 mg (n = 821) donepezil daily. Mean change in circulating white red blood cell counts (RBC) and platelets between 4 weeks before and 3 months after treatment. RBC: P = 0.0453 for no donepezil group vs. 10 mg donepezil group. Platelets: P = 0.0001 for no donepezil group vs. 10 mg donepezil group and P = 0.0001 for 5 mg donepezil group vs. 10 mg donepezil group. One-way ANOVA with Sidak’s post hoc test. Box shows 25th, 50th and 75th percentile but no whiskers or outliers. Blue dot represents the mean. b. Immunofluorescence image of a naive ChatGFP mouse femur. Section was stained with DAPI (blue; cell nucleus). Arrows indicate ChatGFP-positive nerves outside the bone marrow. The experiment was repeated two independent times with a similar result. Scale bar upper panel, 1 mm. All other panels, scale bar, 70 µm. Data are mean ± SEM with individual values for data distribution, *P < 0.05, and ****P < 0.0001.
