Extended Data Fig. 5: Matrix movements on organ surfaces are independent of EN1 + fibroblasts.
From: Neutrophils direct preexisting matrix to initiate repair in damaged tissues
a, Representative immunohistological stainings of organ wounds 24 hours after injury. Quantification of Ly6G+ neutrophils and PDGFR + NCad+ fibroblasts in organ wounds 24 hours post injury. n = six C57BL/6 J WT mice and three independent experiments. Scale bar: 50 µm. Two-tailed Mann–Whitney; * P < 0.05. b, Representative immunohistological stainings of EN1Cre;R26mTmG transgenic mouse organ wounds 24 hours after injury. Skin histology acts as EN1 reporter control, showing EN1+ cells in skin wounds as previously reported12. n = six mice and four independent experiments. Scale bar: 50 µm. c, Representative images of organ surfaces in the presence of N-cadherin inhibitor Exherin post 24 hours after injury. n = four C57BL/6 J WT mice and three independent experiments. Two-tailed Mann–Whitney; NS = not significant. Scale bar: 500 µm.
