Extended Data Fig. 8: Peptide stimulation confirms specificity of αβTCR motifs.
From: SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8+ T cells
Top: example of the gating strategy (B15-specific Jurkat line 1, same as Fig. S4). Left column: unstimulated control. Each row shows stimulation with a single peptide (middle columns), B15 specific TCRs were stimulated with both NQKLIANQF (SARS-CoV-2) peptide and NQKLIANAF (OC43 and HKU1) peptide; Right column: PMA/Ionomycin (positive control). Responsiveness of the Jurkat cell lines was determined using an endogenous NFAT-GFP reporter.
