Extended Data Fig. 1: KSHV ORF45 triggers inflammasome activation.

a,b ASC spike microscopy images (a) and its quantification (b) in GFP-ASC HeLa^WT or HeLa^{IFI16 KO} cells infected by KSHV. Scale bar, 50 μm. n = 4. c IFI16 knockout in HeLa cell by CRISPR-Cas9. d ASC spike microscopy images in GFP-ASC HeLa cells at 48 h after infection with lentiviruses carrying individual KSHV-encoded protein. Scale bar, 50 μm. e,f IL-1β production (e) and LDH release (f) in THP-1 cells infected by KSHV^WT, KSHV^UV, or KSHV^ΔORF45 for 24 h (n = 3). g LDH release of HEK293T cells expressing Tet-vector or Tet-ORF45 treated with 1 μg/ml Dox for 48 h (n = 3). h Fluorescence microscopy images in THP-1 cells with TAT-GFP treatment for 2 h. Scale bar, 50 μm. All data represent three independent experiments and are presented as mean ± s.d. For statistical analysis, b,g two-tailed paired Student’s t test. e,f one-way ANOVA.

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