Extended Data Fig. 2: hNLRP1 is required for ORF45-mediated inflammasome activation.
a Endogenous expressions of hNLRP1, NLRP3, NLRC4, IFI16 and Pyrin in THP-1, BJAB, HEK293T and HeLa cells by immunoblot with indicated antibodies. b,c ASC spike microscopy images (b) and its quantification (c) in GFP-ASC HeLaWT or HeLaNLRP1 KO cells infected by KSHV for 48 h. Scale bar, 50 μm. n = 4. d,e ASC spike microscopy images (d) and its quantification (e) in GFP-ASC HeLa cells infected by lentiviruses carrying ORF45, 3CPro, and vector control for 48 h. Scale bar, 50 μm. n = 3. f-h LDH release (f,g) and IL-1β production (h) in THP-1WT or THP-1NLRP1 KO cells treated with TAT-GFP, TAT-ORF45, TAT-3Cpro (10 μg/ml), or poly I:C (500 ng/ml) for 6 h (n = 3). All data represent three independent experiments and are presented as mean ± s.d. For statistical analysis, c,g,h two-tailed paired Student’s t test. e,f one-way ANOVA.
