Extended Data Fig. 1: Knockdown of NLRP11 impairs NLRP3-mediated cytokine release.
From: NLRP3 licenses NLRP11 for inflammasome activation in human macrophages
(a) Immunoblot for NLRP11, NLRP3 and tubulin loading control using TCL of LPS-primed (200 ng/ml, 4 h) stable control (CtrlKD#1 and CtrlKD#2) and NLRP11 knockdown (KD) THP-1 cells (NLRP11KD#1 and NLRP11KD#2). (b) IL-1β and IL-6 ELISA of SN from CtrlKD#1, CtrlKD#2, NLRP11KD#1 and NLRP11KD#2 cells left untreated or primed with LPS (200 ng ml−1, 4 h) and primed+activated with Nig (5 µM, 30 min), CTB (20 µg ml−1, 6 h) and Silica (200 µg ml−1, 6 h); (n = 3, mean ± s.d.); *p = 0.0121; **p = 0.0068, ***p = 0.0101, ****p = 0.0018. (c) Quantitative real-time PCR of IL1B mRNA levels from CtrlKD and NLRP11KD cells left untreated and primed with Pam3CSK4 (1 μg ml−1, 2 h) and presented as fold induction compared to Ctrl cells (n = 3, mean ± s.d.). (d) Immunoblot for NLRP11 and tubulin loading control using TCL of primary human macrophages either not transfected (Ctrl), transfected with CtrlsiRNA or NLRP11siRNA and primed with LPS (200 ng/ml, 4 h).
