Extended Data Fig. 2: NLRP11 knockout THP-1 cells.
From: NLRP3 licenses NLRP11 for inflammasome activation in human macrophages
(a) PCR amplification and sequencing analysis of Cas9Ctrl, NLRP11KO#1 and NLRP11KO#2 THP-1 cells, showing two distinct deletions of 4 and 172 bp. (b) The predicted amino acid sequence and protein length of the resulting NLRP11 proteins are indicated, with the premature stop marked by an asterisk. (c) Domain architecture of the six predicted NLRP11 isoforms. A red arrowhead marks the 2 gRNA targeting sites. (d) PCR amplification and sequence analysis of Cas9Ctrl, NLRP11KO#1 and NLRP11KO#2 cells showing the 229 bp deletion in the NACHT/NAD. (e) The predicted amino acid sequence and protein length of the resulting NLRP11 proteins are indicated, with the premature stop marked by an asterisk. (f,g) Immunoblot for (f) ASC, NLRP3, Caspase-1 and tubulin loading control using TCL from Cas9Ctrl, NLRP11KO#1, NLRP3KO, ASCKD and CASP1KO cells and (g) caspase-4 and tubulin loading control from TCL of Cas9Ctrl and CASP4KO cells.
