Extended Data Fig. 3
a, FACS analysis validating that Aire protein expression in the entire LN-resident cell compartment is exclusively restricted to a CD45 positive, lineage negative (Lin 1: TCR-β, CD19, Gr1, F4/80, CD11b, CD11c, NK1.1; Lin 2: CD3, B220), NKp46-negative, MHCII positive, Rorγt positive cell subset – previously identified as Aire+ ILC3s. b, Back-gating FACS analysis of Aire-expressing cells identified in a. c, Representative flow cytometry dot plots showing the frequencies of transferred CD45.1+ OT-II T cell (red gate) vs. CD45.1/CD45.2 double positive control T cell (blue gate) populations 2 days after the transfer. d, Statistical analysis of ratios related to b (n = 5 per group, mean ± SD, two-tailed Student’s t-test) of OT-II vs. control T two days after the transfer. e,f, Survival curves of WT (Aire+/+) and knockout (Aire–/–) mice (n ≥ 10 mice per group) on either on NOD (e) and C57Bl/6 (f) genetic background after systemic challenge with live C. albicans. Mice were i.v. injected every second day by heat-killed C. albicans (HKCA) for the duration of three weeks. Subsequently, mice were infected by alive C. albicans and monitored for survival. Long-rank (Mantel-Cox) test was used to calculate the indicated P-value. g,h, Quantitative PCR analysis assessing the presence of C. albicans-specific DNA in the liver (g) and small intestine (h) from Rorc-Cre– Airefl/fl (WT) and Rorc-Cre+Airefl/fl (ILC3ΔAire) mice (n = 6, mean ± SD, two-tailed Student’s t-test). i,j, ELISA assessing the amount of IL-17 (i) or (IL-22) (j) autoantibodies in the sera of 8-week old untreated Aire+/+ vs. Aire–/– mice on NOD and C57Bl/6 (B6) genetic background (n = 6 per group, two-tailed Student’s t-test). Data are shown as mean of optical density ± SD. Data are shown as mean of optical density ± SD. P-value indicators: *** = P-value < 0.0001, ** = P-value < 0.001, ns = not significant.
