Extended Data Fig. 5
a–e, Flow cytometry analysis of C albicans-specific T cells (using Als1-Tet) after oral colonization. Aire whole-body knockout mice (Aire−/−), their wild-type littermates (Aire+/+), WT (Rorc-Cre–Airefl/fl) and ILC3ΔAire (Rorc-Cre+Airefl/fl) were orally colonized by C. albicans and analyzed after 24 hours (left panel) or two weeks (right panel). a, Representative FACS plot of Als1-Tet+ T cells. Cells were isolated from pLNs and spleens (SLO), oral mucosa, esophagus and intestine of mice described above 24 hours (left panel) or 2 weeks (right panel) post C. albicans colonization. Counts of Als1-Tet+ cells are highlighted in red rectangles. b–e, Statistical analysis of the Als1-Tet+ cells counts (n = 6, mean ± SD, two-tailed Student’s t-test in SLO (b), oral mucosa (c), esophagus (d) and small intestine (e) 24 hours (left panel) or 2 weeks (right panel) post C. albicans colonization (n = 6, mean ± SD, two-tailed Student’s t-test). P-value indicators: *** = P-value < 0.0001, ** = P-value < 0.001, * = P-value < 0.05, ns = not significant.
