Fig. 3: Conduits are stretched in the swelling lymph node.
From: Multitier mechanics control stromal adaptations in the swelling lymph node
a, Schematic of STEM tomography acquisition of macerated popliteal lymph node samples (left) and images of the fibrillar collagen of T-zone conduits at a single tilt angle (middle) and a maximum intensity projection crop of a 3D conduit reconstructed from multiple tilting angles (right). b, Representative cropped 3D reconstructions of fibrillar collagen (blue) from macerated conduits at homeostasis (day 0) and inflammation (days 2, 4 and 14) in which the conduit centerline (yellow) and traced fibril segments (gray) are depicted. c, Visual representation of the conduit fibril alignment analysis of an imaged 3D conduit volume. Angles of individual fibril segments (thick colored lines) with the centerline of the conduit (dashed black line) are measured at multiple points along the fibril segment (thin colored lines) and averaged per fibril segment. ɑ1 and ɑ2 indicate measured angles. d, Quantification of conduit fibril alignment with centerline (n = 437, 244, 502 and 478). Data are shown as the mean. Datapoints represent an individual fibril segment. Statistical analysis was performed using the Kruskal-Wallis test. All experiments were repeated independently (three lymph nodes from two mice and two experiments) and data were pooled for each time point. For statistical details, see Supplementary Table 1. NS, not significant. ***P < 0.001, ****P < 0.0001.
