Fig. 5: T-zone reticular cells undergo distributed clonal expansion.
From: Multitier mechanics control stromal adaptations in the swelling lymph node
a, High-resolution confocal volumes of MADM sparse labeled TRCs in homeostasis (day 0) and TRC clusters in inflammation (day 8). Scale bars, 40 µm. b, Quantification of LN volume (left; n = 5, 5 and 5) and number of clusters (right; n = 5, 5 and 5) of light-sheet images from cleared popliteal lymph nodes of Ccl19-Cre MADM-7GT/TG mice in homeostasis (day 0) and inflammation (days 4 and 8). c, Representative images of TRC cluster volumes (randomly colored) and HEVs of an entire LN in homeostasis (day 0) and inflammation (day 4) from experiments as in b. Scale bar, 200 µm. d, Frequency distribution in percentages of TRC per cluster found in observed and simulated data from experiments as in b. Data are depicted as the mean (n = 5, 5 and 5). e, Quantification of the CF per LN from experiments as in b (n = 5, 5 and 5). f, CF plotted as a function of the distance from the nearest HEV from experiments as in b. Data are depicted as the mean ± s.e.m. (n = 5, 5 and 5). g, Correlation matrix of paired variables assessed in the cluster analysis from experiments as in b (n = 15). P values are given and the correlation coefficients are color coded. h, CF plotted as a function of the LN volume from experiments as in b (n = 15). A spline fit was plotted through the datapoints. Data from b and e are depicted as the mean ± s.d. Datapoints from b and e represent a single analyzed LN. Statistical analysis was performed using one-way ANOVA (b; left), Kruskal-Wallis test (b; right, e) and two-tailed Spearman correlation (g). All experiments were repeated independently (≥3 mice and ≥2 experiments). For statistical details, see Supplementary Table 1. **P < 0.01, ***P < 0.001, ****P < 0.0001.
