Extended Data Fig. 5: Sirt1 deficiency potentiates innate antiviral immunity.
a, Schematic diagram of Sirt1 knockout strategy. Lyz2-Cre+Sirt1f/f mice in C57BL/6 N background was generated by targeting of exon 4 of Sirt1 using CRISPR/CAS9. Deletion of exon 4 results in frame shift and disrupts its open reading frame (ORF), leading to the loss of Sirt1 expression. b, Left: immunoblot analysis (IB) of Sirt1 in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages, assessed after immunoprecipitation (IP), was shown. Right: qPCR analysis of Ifnb1, Ifna, Cxcl10 and Ccl5 mRNA in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages infected with SeV for the indicated time points. c, qPCR analysis of Ifnb1, Cxcl10 and Ccl5 mRNA in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages stimulated for indicated time points with 5′-triphosphorylated RNA (5′-ppp RNA). d, qPCR analysis of Ifnb1 and Ifna mRNA in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages transfected with poly(I:C) for indicated time points. Data presented as mean ± s.d., n = 3 biological independent samples and the statistical analysis was performed using two-tailed Student’s t-test (b–d).
