Extended Data Fig. 6: IFN signaling is down-regulated in Sirt1-deficient cells. | Nature Immunology

Extended Data Fig. 6: IFN signaling is down-regulated in Sirt1-deficient cells.

From: Deactylation by SIRT1 enables liquid–liquid phase separation of IRF3/IRF7 in innate antiviral immunity

Extended Data Fig. 6

a, qPCR analysis of Ifnb1 and Ifna mRNA, VSV copy number, and plaque assay of VSV (right), in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages mock infected (PBS) or infected with VSV (MOI, 0.1) for various times (horizontal axis). b, qPCR analysis of Ifnb1 and Ifna mRNA, copy number of HSV-1 genomic DNA and plaque assay of HSV-1 in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f peritoneal macrophages mock infected (PBS) or infected with HSV-1 (MOI, 10) for various time courses (horizontal axis). c, qPCR analysis of Ifnb1 and Ifna mRNA in Lyz2-Cre−Sirt1f/f and Lyz2-Cre+Sirt1f/f Bone Marrow Derived Macrophages (BMDMs) treated with SeV (Upper), poly(I:C) (Middle) or HSV-1 (Lower) respectively for indicated time points. d, qPCR analysis of Ifnb1 and Ifna mRNA in wild-type and Sirt1−/−MEF cells treated with SeV (upper), poly(I:C) (middle) or HSV-1 (lower) (MOI, 10) respectively for indicated time points. Data are presented as mean ± s.d.; n = 3 biological independent samples and the statistical analysis was performed using two-tailed Student’s t-test (a–d).

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