Extended Data Fig. 9: STACs promote SIRT1-mediated deacetylation of IRF3/7 and elevate the innate antiviral response.
a, Immunoblot (IB) of the total cell lysate (TCL) and immunoprecipitates (IP) derived from Sirt1+/+ and Sirt1−/− MEFs treated for 12 h with control DMSO (−), SRT501 (50 μM) or SRT2183 (10 μM). b, ChIP in Sirt1+/+ and Sirt1−/− MEF cells pre-treated with control DMSO (−), SRT501 (50 μM) or SRT2183 (10 μM), followed by infection for 12 h with SeV. c–d, qPCR analysis of Ifnb1 (c, left), Ifna (c, right) and VSV mRNA (d) in spleen, liver and lung from mice as in Fig. 8g. e, Microscopy of hematoxylin-and-eosin (H&E)-stained lung sections from mice as in Fig. 8g. Scale bar, 100 µm. n = 3. f, Immunofluorescence microscopy of IRF3 (upper), IRF7 (lower) and DAPI staining in liver from mice as in Fig. 8g. n = 3. Data are representative of three independent experiments (a). n = 3 (b, e, f) or 4 (c, d) independent biological replicates. Mean ± s.d., statistical analysis was performed using two-tailed Student’s t-test (b–d).
