Fig. 1: The FRC network is under mechanical tension in steady-state LNs.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a, LN tile scan (left), paracortex (right) maximum z-projection of PDPN (FRCs, yellow), and CD3 (T cells, blue). Scale bars, 500 µm (left) and 25 µm (right). b, FRC network structure and T cell compaction in vibratome slices of PDPN (FRCs), CD3 (T cells), and DAPI (nuclei). Scale bars, 50 µm (left) and 10 µm (right). c, PDGFRα+mGFP+ (FRCs) ablation region of interest (ROI) (white box) (left) and cut site (red dotted line) (middle). Scale bars, 50 µm. Right: Recoil displacement (arrowheads) with pre- (green) and postcut (magenta) overlay (right). Scale bar, 10 µm. d, Initial recoil velocity (µm s−1). Each point represents an ablation. Box plot indicates median, interquartile range, and minimum/maximum. e, Individual recoil curves (black) compared to the mean (blue). d,e, n = 30 individual ablations over 10 LNs. f, Paracortical steady-state FRCs of perlecan (matrix; purple), PDPN (FRC; yellow), phalloidin (F-actin; blue), and pMLC (red). Asterisk and arrowheads indicate F-actin cables. Orthogonal views (yellow dotted line, yz axis, 10 μm depth). Representative image of three independent experiments. Scale bars, 10 µm.
