Extended Data Fig. 3: Y27 treatment of lymph node slices reduce tension in the FRC network.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a-c) Recoil curves show displacement over time (mean ± SEM) (top panel). Individual recoil curves of control (middle) and Y27 treated LNs (bottom). n = individual ablation where Day 0 (n = 54), Day 0 + Y27 (n = 43), Day 3 (n = 18), Day 3 + Y27 (n = 20), Day 5 (n = 19), Day 5 + Y27 (n = 15) over 3 independent experiments. d) Membrane GFP visualizes the FRC network in the ex vivo lymph node slice. Y27 treatment has no effect on the FRC network connectivity. Scale bar, 50 μm. This figure supports Fig. 3.
