Extended Data Fig. 6: Flow cytometry of PdgframGFPΔPDPN fibroblasts.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a) Example PDPN contour plots for GFP+ FRCs from control and PdgframGFPΔPDPN LNs. b) Representative histograms of surface protein expression for PDPN in control and PdgframGFPΔPDPN LNs 5 Days post CFA/OVA. c) Fold change in PDPN expression (gMFI) in FRCs (GFP+ CD31−, CD140a+ CD31−). Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons. ****p = 1.00E^−6, ***p = 0.000143, 0.0004, **p = 0.0084, *p = 0.0145. d) Fold change in PDPN expression (gMFI) for LECs (PDPN+ CD31+). Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons. **p = 0.0033. c, d) n = individual LNs where (n = 12), Day 0ΔPDPN (n = 12), Day 5 (n = 6), Day 5ΔPDPN(n = 8) over 2 independent experiments. This figure supports Figs. 6, 7.
