Extended Data Fig. 8: T-cell activation and proliferation is not altered by PDPN deletion or Y27 treatment.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a) Representative flow cytometric gating and cell number comparing control and PdgframGFPΔPDPN CD3+ CD4+ CD25+, CD3+ CD8+ CD25+ population. Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****p = 1.00E^−6, **p = 0.0046, 0.0063. n = individual LNs where Day 0 (n = 12), Day 0ΔPDPN (n = 12), Day 5 (n = 7), Day 5ΔPDPN(n = 8) over 2 independent experiments. b) Representative images of Ki67+ CD3+ staining of PBS or Y27 treated lymph nodes. Scale bar, 100 μm. c) Quantification of Ki67+ CD3+ T cells per area. Box plot indicates median, interquartile range and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****p = 1.00E^−6. n = individual image ROIs where Day 0 (n = 13), Day 0 + Y27 (n = 15), Day 5 (n = 18), Day 5 + Y27 (n = 20) from 3 independent LNs. This figure supports Figs. 7 and 8.
