Fig. 2: FRCs adapt to changing external forces throughout immune challenge.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a, LN mass change after IFA/OVA immunization. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons, ****P = 1.00 × 10−6. Each point represents one LN at day 0 (n = 10), day 3 (n = 11), and day 5 (n = 12). b, T cell packing in the LN during inflammation for PDPN (FRCs, yellow), CD3 (T cells, blue), and DAPI (nuclei, gray). Scale bars, 25 µm. c, Quantification of CD3+ T cell nuclei per 100 µm2. Box plot indicates median, interquartile range, and minimum/maximum. Dotted line represents median of day 0. One-way ANOVA with Tukey’s multiple comparisons, *P = 0.0362, ****P = 0.0003. n indicates individual image ROI on day 0 (n = 17), day 3 (n = 15), and day 5 (n = 20) from three independent LNs. d, FRC gap analysis during inflammation. PDPN (FRCs), binary, and circle overlay. Scale bars, 50 µm. e, Quantification of circle radius. Box plot indicates median, interquartile range, and minimum/maximum. Dotted line represents median of day 0. One-way ANOVA with Tukey’s multiple comparisons, ****P = 2.00 × 10−6. Each point represents a circle radius. n indicates individual image ROI on day 0 (n = 12), day 3 (n = 15), and day 5 (n = 15) from three independent LNs. f, Laser ablation of the FRC network throughout inflammation. PDGFRα+mGFP+ (FRCs) ablation ROI (white box) and cut site (red dotted line). Scale bars, 50 µm. Recoil displacement (arrowheads) with pre- (green) and postcut (magenta) overlay. Scale bars, 10 µm. g, Recoil curves of network displacement (μm) (mean ± standard error of the mean (s.e.m.)). h, Initial recoil velocity (μm s−1) after IFA/OVA immunization. Box plot indicates median, interquartile range, and minimum/maximum. Kruskal–Wallis test with Dunnett’s test, *P < 0.05. g,h, n indicates an ablation on day 0 (n = 30), day 3 (n = 37), and day 5 (n = 32) for three independent experiments.
