Fig. 7: Loss of FRC mechanical sensitivity constrains effector T cell populations in vivo.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a, LN mass. b, Cellularity. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10−6, ***P = 0.000567, **P = 0.002087. n indicates LNs at day 0 (n = 12), day 0ΔPDPN (n = 12), day 5 (n ≥ 6), and day 5ΔPDPN(n = 8) over two independent experiments. c–e, CD45+, CD45−, CD19+ and CD3+ cell numbers. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10−6, ***P < 0.001, **P = 0.00989, *P = 0.027535. f, Ratio of CD19+ and CD3+ cell numbers. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10−6. Each point represents one LN. g, Flow cytometric gating. Representative dot plots and percentages of CD45+, CD45−, CD19+, CD3+ and CD3+ CD4+ and CD3+ CD8+ subpopulations. h, Representative flow cytometric gating comparing control and PdgframGFPΔPDPN CD3+ CD4+, CD3+ CD8+ subpopulations. i,j, CD3+ CD4+ and CD3+ CD8+ subpopulation cell numbers. Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10−6, **P = 0.0058, *P < 0.05. Each point represents one LN. k–m, Stromal cell numbers for blood endothelial cells (BECs) (k), lymphatic endothelial cells (LECs) (l), and GFP+ and CD140α+ FRCs (m). Box plots indicate median, interquartile range, and minimum/maximum. One-way ANOVA with Tukey’s multiple comparisons, ****P = 1.00 × 10−6, **P = 0.0043, *P < 0.05.
