Extended Data Fig. 1: Flow cytometry of PDGFRαmGFP fibroblasts, ex vivo laser ablation and immunization.
From: Lymph node homeostasis and adaptation to immune challenge resolved by fibroblast network mechanics
a-b) Flow cytometric gating strategy and representative dot plots and percentages of a) CD45− stroma cells and b) subpopulations. Fibroblastic reticular cells (FRC, PDPN+CD31−), blood endothelial cells (BEC, PDPN−CD31+), lymphatic endothelial cells (LEC, PDPN+CD31+). Red arrow shows GFP+ FRCs c) Representative gating of GFP+ Fibroblasts. d) A membrane-targeted GFP molecule is driven under the PDGFRα promoter. e) LN nodes were embedded in low melt agarose and sliced at 200 μm thickness before being secured by cover glass for imaging. f) IFA/OVA is used as model immunization with inguinal draining (dr) and non-draining (nDr) lymph nodes (LNs) harvested day 3 or day 5 post immunization (right). This figure supports Figs. 1–2.
