Extended Data Fig. 3: Jmjd1c expressed in B cells was dispensable for T cell helper function, but was required for antibody production.
From: Jmjd1c demethylates STAT3 to restrain plasma cell differentiation and rheumatoid arthritis
a, Left, representative flow cytometry plots of splenic CD4+ T cell subsets: CD4+ naïve T (CD44-CD62L+) and CD4+ T effector memory (CD44+CD62L–) cells from Jmjd1c+/+ Mb1Cre+ (n=4) and Jmjd1cfl/fl Mb1Cre+ mice (n=5). Right, frequency of the T cell populations. b, Splenocytes from Jmjd1c+/+ Mb1Cre+ and Jmjd1cfl/fl Mb1Cre+ mice were cultured with or without 1 μg/ml synthetic Eα52–68 peptide for 1 h, and then the levels MHC-II-Ea complex on B cells were measured with Y-Ae antibody by FACS. c and d, Jmjd1c+/+ Mb1Cre+ and Jmjd1cfl/fl Mb1Cre+ mice were induced for the collagen arthritis. (C) Left, representative flow cytometry plots of Th1 (CD4+IFN-γ+) cells from inguinal lymph nodes. Right, number of the Th1 cells. (D) ELISA of anti- collagen IgG2b (left), IgG2c (middle) and IgG1 (right) from serial serum samples. Data are means ± SEM. Student’s t tests were used for analyses in (a) and (c) (NS, not significant). Two-way ANOVA were used for (d) (***p < 0.001). All data are representative of 3 individual experiments.
