Extended Data Fig. 5: Increased Stat3 phosphorylation and antibody production in Jmjd1cfl/fl CD19Cre+ mice.
From: Jmjd1c demethylates STAT3 to restrain plasma cell differentiation and rheumatoid arthritis
a, B cells from Jmjd1c+/+ CD19Cre+ and Jmjd1cfl/fl CD19Cre+ mice were stimulated with anti-IgM (left) or IL-6 (right) for 16 h. Expression of p-Stat3 was subsequently analyzed by immunoblot. Blots were probed against total Stat3, phosphorylated Stat3 (Y705) and β-actin. b, ELISA of NP-specific IgM and IgG from serum of Jmjd1c+/+ CD19Cre+ and Jmjd1cfl/fl CD19Cre+ mice immunized with NP-Ficoll for 7 days. c, ELISA of NP-specific IgG1 from serum of Jmjd1c+/+ CD19Cre+ and Jmjd1cfl/fl CD19Cre+ mice immunized with NP-KLH at day 7, 14, 21. Data are means ± SEM. Two-way ANOVA were used (***p < 0.001). Data are representative of 2 individual experiments.
