Extended Data Fig. 1: Normal B cell development in the absence of Jmjd1c.
From: Jmjd1c demethylates STAT3 to restrain plasma cell differentiation and rheumatoid arthritis
a, The Jmjd1c gene conditional targeting strategy. Exon 9 and 10 was floxed and deleted by crossing to Cre+ mouse strain. Enzymatic JmjC domain was encoded by Exon 21-26. b, Left, flow cytometric analysis showing expression of CD11b, B220, CD43, BP-1, CD24, IgM and IgD in bone marrow cells from Jmjd1c+/+ Mb1Cre+ (n=4) and Jmjd1cfl/fl Mb1Cre+ mice (n=4). Right, absolute numbers of B220+CD43+ and B220+CD43– bone marrow cells per femur in each developmental stage. c,Left, expression of B220, IgD, IgM, AA4.1, CD23, and CD21 in splenocytes from Jmjd1c+/+ Mb1Cre+ (n=5) and Jmjd1cfl/fl Mb1Cre+ (n=5) mice. Right, absolute number of B220+ splenocytes in each developmental stage. Data are means ± SEM. Student’s t tests were used (NS, not significant).
