Extended Data Fig. 4: Inhibiting recycling using dominant negative Rab11 impairs CD86 transendocytosis.
a). CTLA-4+ HeLa cells were transiently transfected with dominant negative (DN) Rab11-GTPase or empty vector backbone and the impact on transendocytosis assessed using CHO mCherry-ligand uptake at 20h assessed by flow cytometry. Statistical significance was determined by 2-way ANOVA with Sidak’s multiple comparison correction, ****P < 0.0001. All data are presented as mean ± s.d. and show individual data points from 5 independent experiments. b). Impact of Kless and LRBA on CTLA-4 degradation. Comparison of CTLA-4 sensitivity to BafA in WT, Kless and LRBA knockout Jurkat T cells. CTLA-4 degradation in the steady state was estimated by the impact of treatment with BafA on the staining for total CTLA-4 expression.
