Fig. 7: CTLA-4 Arg70Gln is unable to regulate a CD86-driven T cell response. | Nature Immunology

Fig. 7: CTLA-4 Arg70Gln is unable to regulate a CD86-driven T cell response.

From: Differences in CD80 and CD86 transendocytosis reveal CD86 as a key target for CTLA-4 immune regulation

Fig. 7

a,b, CTFR-labeled CD86-expressing (a) or CD80-expressing (b) DG-75 B cells exposed to Jurkat T cells expressing no CTLA-4, CTLA-4 Arg70Gln or CTLA-4 WT overnight in a TE assay. TE of GFP–ligand is shown in the left-hand column. After TE, B cells were then used to co-stimulate T cell proliferation of CTV-labeled human CD4+CD25− T cells (representative histograms). T cell receptor stimulation was provided by soluble anti-CD3 antibody and proliferative responses measured by flow cytometry at day 5 for CTV dilution. c, Quantification of the experiment shown in a and b, using data from six individual donors, showing the percentage of T cells in the initial culture that entered cell division. The statistical significance was determined by two-way ANOVA with Sidak’s multiple comparison correction: ***P < 0.001, ****P < 0.0001 All data are presented as mean ± s.d. and show individual data points from six biologically independent samples.

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