Extended Data Fig. 7: Disruption of the 3D architecture of the Id2 locus reduces ILC2 numbers in the lung but does not drastically change other immune cell populations at steady state.
From: Multiscale 3D genome organization underlies ILC2 ontogenesis and allergic airway inflammation
a, Representative flow cytometry gating strategy used for the identification of immune cell populations in digested lungs or in bronchoalveolar lavage fluid (BALF) at steady state or after 16 days of house dust mite (HDM) challenge in LCR1+/+ and LCR1−/− animals. b, Quantification of frequencies among CD45+ cells and absolute numbers of lung parenchyma ILC2s in LCR1+/+ and LCR1−/− mice at steady state, (n = 8 LCR1+/+ and 8 LCR1−/−). Data are representative of one experiment that was repeated five times. Error bars = SEM; and p-values: ns = not significant, * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.0005 (Mann-Whitney U two-tailed test). c, Quantification of total number of cells of the indicated immune cell populations in Extended Data Fig. 7a in the lung parenchyma of LCR1+/+ and LCR1−/− mice at steady state, (n = 12 LCR1+/+ and 12 LCR1−/−). Data are a pool of two independent experiments, except CD4+ and CD8+ TRM which is a representative experiment. (n = 8 LCR1+/+ and 8 LCR1−/−). Error bars = SEM; and p-values: ns = not significant, * = p ≤ 0.05 (Mann-Whitney U two-tailed test or Two-way ANOVA with multiple comparison and Bonferroni correction).
