Extended Data Fig. 1: Comparison of accessibility between the MNK3 cell line and primary ILC3.
From: Multiscale 3D genome organization underlies ILC2 ontogenesis and allergic airway inflammation
a, Venn diagram representation of the differential chromatin accessibility between ATAC-seq peaks of MNK3 and small intestine (SI) CD4+ ILC3, and SI NCR+ ILC3 cells. Absolute numbers of peaks differentially open (red) or closed (blue) in MNK3, or with similar accessibility (gray) in all samples, are represented. b, View of the chromatin accessibility (ATAC-seq signal) at the Rorc, Il17a, Il17f, Il22 and Il23r loci in small intestine (SI) CD4+ ILC3, SI NCR+ ILC3, and MNK3 cells. c, Venn diagram representation of the differential accessibility analysis results in the region displayed in the right panel. d, View of the chromatin accessibility (ATAC-seq signal) at the Id2 locus in SI CD4+ ILC3, SI NCR+ ILC3, and MNK3 cells. Absolute numbers of peaks differentially open (red) or closed (blue) in MNK3, or with similar accessibility (gray) in all samples, are shown. e, Representative flow cytometry plots showing cell viability and the expression of ILC3-specific markers (IL-22, GM-CSF, CD90.2, RORγt, IL-17 and ID2) by MNK3 cells after stimulation with IL-2, IL-23, and IL-1β. The red line represents activated MNK3 cells and the black line represents unstained MNK3 cells.
